Anti-Inflammatory Action of Indonesian Black Garlic (IBG) Ethanol Extracts in LPS-stimulated RAW 264.7 Macrophage Cells
| Autor: | Laurentia Adinda Pratidina, Seong Gu Hwang, Novita Wijayanti |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Indonesian Journal of Pharmacy, Pp 201-208 (2021) |
| Druh dokumentu: | article |
| ISSN: | 2338-9427 2338-9486 |
| DOI: | 10.22146/ijp.1658 |
| Popis: | Indonesian Black Garlic (IBG) ethanol extracts is a new type of fresh garlic product, produced at high temperature and humidity. IBG has active antioxidant activity. Thy study aimed to evaluate the anti-inflammatory actions of IBG in RAW 264.7 macrophage cells. The cells were cultures and treated with different concentrations of IBG (50 to 1000 mg/mL). The cell counting kit-8 assay showed that IBG was not toxic up to 400 mg/mL. IBG significantly decreased the expression of inflammatory mediators in LPS-stimulated RAW 264.7 macrophage cells. Futhermore, IBG is shown to inhibit pro-inflammatory cytokines, this is evident from the decreased expressions of iNOS, COX-2, IL-6, NF-kB, and TNF-α in mRNA expressions and also on protein level. The results suggest that IBG can be used to prevent or cure inflammation-related diseases. |
| Databáze: | Directory of Open Access Journals |
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