HBD-2 interactions with erythrocyte membranes in vitro
| Autor: | M. V. Makarenko, D. O. Semeniuk, I. O. Starenka, A. P. Pogribna, I. V. Sokol, L. I. Martinova, D. O. Govsieiev |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | The Ukrainian Biochemical Journal, Vol 91, Iss 1, Pp 65-73 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2409-4943 2413-5003 |
| DOI: | 10.15407/ubj91.01.065 |
| Popis: | HBD-2 – a member of β-defensin family of antimicrobial peptides – is known to permeabilize cell membranes of susceptible cells, but the mechanism of such interactions is poorly understood. In our study, we have used a hemolytic model to explore the kinetic properties of HBD-2 interactions with membranes of human erythrocytes. We ran hemolytic assays with a wide range of both HBD-2 and erythrocyte concentrations, as well as varying pH values, incubation times, and osmotic strengths; each in the presence or the absence of inhibitory substances such as proteins and salts. The results show that HBD-2 cell membrane permeabilization is both dose- and time-dependent (with plateau effect observed in each case), and inversely dependent on erythrocyte concentration. HBD-2 interactions with cell membranes highly depend on pH value and the presence of inhibitors but are not affected by tested osmotic strength range. Our findings suggest that interactions of HBD-2 with cell membranes are mainly electrostatic in nature and are limited by released cell content. We have developed a speculative model of such interaction based on our results. |
| Databáze: | Directory of Open Access Journals |
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