| Autor: |
Jia-Rong Jheng, Chung-Fan Hsieh, Yu-Hsiu Chang, Jin-Yuan Ho, Wen-Fang Tang, Zi-Yi Chen, Chien-Jou Liu, Ta-Jen Lin, Li-Yu Huang, Jyh-Haur Chern, Jim-Tong Horng |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Microbiology, Immunology and Infection, Vol 55, Iss 4, Pp 598-610 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1684-1182 |
| DOI: |
10.1016/j.jmii.2022.04.012 |
| Popis: |
Background: The purpose of this study was to examine the in vivo activity of rosmarinic acid (RA) – a phytochemical with antioxidant, anti-inflammatory, and antiviral properties – against influenza virus (IAV). An antibody-based kinase array and different in vitro functional assays were also applied to identify the mechanistic underpinnings by which RA may exert its anti-IAV activity. Methods: We initially examined the potential efficacy of RA using an in vivo mouse model. A time-of-addition assay and an antibody-based kinase array were subsequently applied to investigate mechanism-of-action targets for RA. The hemagglutination inhibition assay, neuraminidase inhibition assay, and cellular entry assay were also performed. Results: RA increased survival and prevented body weight loss in IAV-infected mice. In vitro experiments revealed that RA inhibited different IAV viruses – including oseltamivir-resistant strains. From a mechanistic point of view, RA downregulated the GSK3β and Akt signaling pathways – which are known to facilitate IAV entry and replication into host cells. Conclusions: RA has promising preclinical efficacy against IAV, primarily by interfering with the GSK3β and Akt signaling pathways. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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