| Autor: |
Peshala Gunasekara, S. M. Handunnetti, Sunil Premawansa, E. W. R. A. Witharana, Indra P. Ratnayake, Pradeep Kaluarachchi, Chandima Karunatilake, R. K. S. Dias, G. A. S. Premakumara, W. M. D. K. Dasanayake, Suranjith L. Seneviratne, Rajiva de Silva |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Clinical and Molecular Allergy, Vol 20, Iss 1, Pp 1-10 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1476-7961 |
| DOI: |
10.1186/s12948-022-00178-9 |
| Popis: |
Abstract Background Allergy to Apis dorsata (Giant Asian Honeybee) venom is the commonest insect allergy in Sri Lanka and South East Asia. However, laboratory diagnosis is difficult as the pure venom and diagnostic reagents are not commercially available. Objective This study assessed the use of four recombinant allergens of A. mellifera venom and the passive basophil activation test in the diagnosis of A. dorsata venom anaphylaxis. Methods Serum IgE levels to four recombinant allergens of A. mellifera, rApi m 1, 2, 5 and 10 were assessed and compared with serum IgE to the crude venom of A. mellifera or V. vulgaris by Phadia ImmunoCAP, in patients who developed anaphylaxis to A. dorsata stings. Basophil activation in response to venom of A. dorsata or V. affinis was assessed using a passive basophil activation test. Association of the severity of the reaction with basophil activation was compared. Results rApi m 1 and 10 combinedly had significant correlation (r = 0.722; p |
| Databáze: |
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