| Autor: |
Sarah V Maifeld, Bodrey Ro, Hoyin Mok, Marla Chu, Li Yu, Ryan Yamagata, Tansy Leonardson, Vera Chio, Bandita Parhy, Samuel Park, Marcia Carlson, Shushil Machhi, Nancy Ulbrandt, Ann R Falsey, Edward E Walsh, C Kathy Wang, Mark T Esser, Fengrong Zuo |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
PLoS ONE, Vol 11, Iss 4, p e0153019 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| DOI: |
10.1371/journal.pone.0153019 |
| Popis: |
Sensitive and precise serology assays are needed to measure the humoral response to antigens of respiratory syncytial virus (RSV) following natural infection or vaccination. We developed and evaluated a collection of electrochemiluminescent (ECL) serology assays using four RSV antigens (F, N, Ga and Gb). To assess the merits of ECL technology, the four ECL serology assays were evaluated using a well-characterized "gold standard" panel of acute and convalescent serum samples from fifty-nine RSV-positive and thirty RSV-negative elderly subjects (≥65 years old). The combined results from the four ECL assays demonstrated good concordance to the "gold standard" diagnosis, reaching 95% diagnostic sensitivity and 100% diagnostic specificity. Additionally, a combination of ECL assays provided higher diagnostic sensitivity than a commercially available diagnostic ELISA or cell-based microneutralization assay. In summary, these data demonstrate the advantages of using ECL-based serology assays and highlight their use as a sensitive diagnostic approach to detect recent RSV infection in an elderly population. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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