A Novel Report on Killer Yeast Strains Identification Methods
| Autor: | Najwa M.J.A. Abu-Mejdad, Adnan I. Al-Badran, Abdullah H. Al-Saadoon |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ, Vol 33, Iss 1 (2020) |
| Druh dokumentu: | article |
| ISSN: | 25200860 1814-5868 2520-0860 |
| DOI: | 10.37077/25200860.2020.32.1.04 |
| Popis: | This study was conducted to isolate and identify killer yeasts from soil samples that collected from different locations in Basrah and Dhi-Qar provinces. Seventy-five soil samples were collected from different areas, including sandy, arable, surface sediment and uncultivated soil, using dilution methods to cultivate a serial dilution of each soil sample. The results showed that a 112 isolates were identified biochemically using VITEK system and molecularly using internal transcribed spacer (ITS1- 5.8S-ITS2) marker. The molecular identification provided fast and precise identification results for the 112 isolates, whereas the VITEK test resulted low identification efficiency (8.2% were accurate and 91.8 % were not). The Diazonium blue B salts produced a good colour reaction in distinguishing between ascomycetes and basidiomycetes. The PCR was more accurate in identification of killer yeasts compared to the VITEK system. |
| Databáze: | Directory of Open Access Journals |
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