Contribution of smFRET to Chromatin Research

Autor: Bhaswati Sengupta, Mai Huynh
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Biophysica, Vol 3, Iss 1, Pp 93-108 (2023)
Druh dokumentu: article
ISSN: 2673-4125
DOI: 10.3390/biophysica3010007
Popis: Chromatins are structural components of chromosomes and consist of DNA and histone proteins. The structure, dynamics, and function of chromatins are important in regulating genetic processes. Several different experimental and theoretical tools have been employed to understand chromatins better. In this review, we will focus on the literatures engrossed in understanding of chromatins using single-molecule Förster resonance energy transfer (smFRET). smFRET is a single-molecule fluorescence microscopic technique that can furnish information regarding the distance between two points in space. This has been utilized to efficiently unveil the structural details of chromatins.
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