Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing

Autor:	Wenqi Wu, Zeyan Shi, Zhongyuan Tang, Huiqun Li, Xiaoke Huang, Xiaolin Liang, Jing Li, Yibin Yao, Weihua Zhao, Meiqing Wu, Jun Luo, Zhenfang Liu
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	Single-cell RNA sequencing Normal karyotype acute myeloid leukemia Heterogeneity Leukemia stem cell Diseases of the blood and blood-forming organs RC633-647.5 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Zdroj:	Experimental Hematology & Oncology, Vol 12, Iss 1, Pp 1-16 (2023)
Druh dokumentu:	article
ISSN:	2162-3619
DOI:	10.1186/s40164-023-00391-5
Popis:	Abstract Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were obtained. In addition, we identified a distinct LSC-like cluster with possible biomarkers in NK-AML (M4/M5) and verified 6 genes using qRT‒PCR and bioinformatic analyses. In conclusion, we utilized single-cell technologies to provide an atlas of NK-AML (M4/M5) cell heterogeneity, composition, and biomarkers with implications for precision medicine and targeted therapies.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/ed386b3963bd4341bef93eade0934ebe Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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