| Autor: |
M. El-Azzi, A. Sleiman, A. Abou Fayad, I. Kassem, R. Bawazeer, L. Okdah, M. Doumith, M. Alghoribi, G. Matar |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
International Journal of Infectious Diseases, Vol 116, Iss , Pp S3- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1201-9712 |
| DOI: |
10.1016/j.ijid.2021.12.007 |
| Popis: |
Purpose: To investigate the acquired resistome in 18 colistin resistant Escherichia coli isolated from different poultry farms in Lebanon, analyze Inc plasmids associated with mcr, and assess potential transmission to humans. Methods & Materials: A total of 18 E. coli were recovered from poultry feces collected from poultry farms in Lebanon. BMD assay was performed to determine the antimicrobial resistance profiles. Whole-genome sequencing highlighted genetic resistance determinants. Transformation confirmed transmissibility of mcr. Results: BMD results showed that all the 18 isolates were colistin resistant. Transformation confirmed that the plasmids carrying mcr-1 were transmissible. Sequencing showed that ST1140 was the most prevalent. The most frequent resistance determinants were tetA and floR. A total of 15 different plasmid replicon types were identified. The mcr-1 was predominantly located on IncX4 plasmids. Additionally, two isolates harbored the IncI2-type self-conjugative and IncI2-type mcr-1-harboring plasmids respectively. Conclusion: The findings show that mcr was the most prevalent resistance determinant in multi-drug resistant E. coli isolated poultry farms in Lebanon. The occurrence of mcr on mobile plasmids highlights a risk of transmission to humans. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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