Vitreous humour as a potential DNA source for postmortem human identification.

Autor: Renata Zbiec, Magdalena Spólnicka, Witold Pepiński, Anna Niemcunowicz-Janica, Ireneusz Stołyszewski, Jerzy Janica
Jazyk: angličtina
Rok vydání: 2007
Předmět:
Zdroj: Folia Histochemica et Cytobiologica, Vol 45, Iss 2, Pp 135-136 (2007)
Druh dokumentu: article
ISSN: 0239-8508
1897-5631
DOI: 10.5603/4543
Popis: PURPOSEThe aim of this study was the assessment of vitreous humor as a potential DNA for forensic human postmortem identification.MATERIAL AND METHODSVitreous humor samples were collected using two alternative approaches from 25 corpses of either sex during autopsies. DNA was extracted by standard organic method. Recovered DNA was quantitiated fluorometrically. AmpFlSTR SGM Plus kit and ABI 310 Genetic Analyzer (Applera) were used to obtain genetic profiles.RESULTSDifferent DNA yields were quantitated in vitreous body depending on cause of death and sampling approach.CONCLUSIONVitreous humor is a potential DNA for forensic human postmortem identification depending on a sampling method used.
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