A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device

Autor: Qiushu Chen, Qi Meng, Yuzhe Liu, Xiangan Long, Yawei Kong, Longfang Yao, Liwen Chen, Chuanyong Wu, Kaiqin Chu, Lan Mi, Jiong Ma
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Innovative Optical Health Sciences, Vol 16, Iss 03 (2023)
Druh dokumentu: article
ISSN: 17935458
1793-7205
1793-5458
DOI: 10.1142/S1793545822440060
Popis: Microfluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of-field (EDoF) phase modulator to the optical system, the entire depth of the microfluidic channel can be covered in one image without any refocusing process, ensuring that 95% of the particles in the droplet are captured within three shots together with shaking processes. With this system, suspension droplets are generated and droplets containing only one yeast cell can be recognized, then each single cell is cultured in the array of the chip. By observing their growth in cell numbers and the green fluorescence protein (GFP) production via fluorescence imaging, the single cell with the highest production can be identified. The results have proved the heterogeneity of yeast cells, and showed that the combined system can be applied for rapid single-cell sorting, cultivation, and analysis.
Databáze: Directory of Open Access Journals
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