Berberine nanomicelles attenuate cirrhotic cardiomyopathy in rats: Possible involvement of the NO-cGMP signaling
| Autor: | Nahid Fakhraei, Seyyedeh Elaheh Mousavi, Mahsa Sadeghi Adl, Seyed Pouyan Pishva, Fatemeh Tabarsa, Seyed Mahdi Rezayat, Amir Rashidian, Ahmad Reza Dehpour |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Nanomedicine Journal, Vol 7, Iss 4, Pp 229-307 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2322-3049 2322-5904 |
| DOI: | 10.22038/nmj.2020.07.00006 |
| Popis: | Objective(s): In cirrhotic cardiomyopathy, a rise in pro-inflammatory cytokines results in the up-regulation of inducible nitric oxide synthase (iNOS), and the overproductions of nitric oxide (NO) and cyclic guanosine 3’, 5’ monophosphate (cGMP). Berberine (BBR), an isoquinoline-derived alkaloid isolated from Rhizoma coptidis, possesses anti-inflammatory, anti-oxidative, and cardioprotective properties. In this study, the effect of BBR-loaded micelles in a rat model of cirrhotic cardiomyopathy resulted from bile duct-ligation (BDL) was examined. Further, a possible role for NO-cGMP signaling was clarified. Materials and Methods: Cirrhotic rats were orally treated with BBR-loaded micelles (50 mg/kg), free BBR (50 and 100 mg/kg) and silymarin (100 mg/kg). A selective iNOS inhibitor, aminoguanidine (AG) 100 mg/kg, i.p., was administered. iNOS expression and nitrite concentration were calculated using immunohistochemistry (IHC) and Griess reagent methods, respectively. Besides, ventricular tumor necrosis factor-alpha (TNF-α), cGMP, and serum interleukin -1beta (IL-1β) were measured using ELISA kits. Results: TNF-α and IL-1β, nitrite, cGMP, and the expression of iNOS increased significantly in BDL rats. However, BBR (100 mg/kg), nanoBBR (50 mg/kg), and silymarin markedly lowered the levels of these markers. Notably, AG increased the nanoBBR effect.Conclusion: This cardioprotective effect of nanoBBR probably mediated at least in part by down-regulations of the NO-cGMP pathway, and the inflammatory mediators. |
| Databáze: | Directory of Open Access Journals |
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