| Autor: |
Mustapha A. Ahmed, Elvis O. Ameyaw, Francis Ackah-Armah, Desmond O. Acheampong, Benjamin Amoani, Paulina Ampomah, Emmanuel A. Adakudugu, Christian K. Adokoh |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Traditional and Complementary Medicine, Vol 12, Iss 4, Pp 391-401 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2225-4110 |
| DOI: |
10.1016/j.jtcme.2021.11.004 |
| Popis: |
Background: The emergence of widespread drug-resistant strains of the malaria parasites militates against strives for more potent antimalarial drugs. Aim: The present study evaluated the antimalarial activity of A. africana ethanolic crude extract in vitro and in vivo against Plasmodium berghei -infected mice in anticipation of acquiring scientific evidence for it used by mangrove dwellers to treat malaria in Ghana. Methodology: The pulverized dried leaves were extracted with 70% ethanol (v/v) and screened for phytochemicals using standard protocols. The in vitro antimalarial activity was investigated against chloroquine-sensitive Plasmodium falciparum (Pf3D7 clones), MRA-102, Lot:70032033, via SYBR® Green I fluorescent assay method using positive control Artesunate (50–1.56 × 10−3 μg/mL). In the in vivo studies, doses (200–1500 mg/kg) of AAE were used in the 4-day suppressive and curative tests, using P. berghei-infected mice. Artemether/lumefantrine (1.14 mg/kg) and normal saline were used as positive and negative control respectively. Results: The phytochemical analysis revealed the presence of alkaloids, saponins, flavonoids, glycosides, tannins, terpenoids and phytosterols. The extract showed an IC50 of 49.30 ± 4.40 μg/mL in vitro and demonstrated complete parasite clearance at dose 1500 mg/kg in vivo with a suppressive activity of 100% (p 5000 mg/kg in mice. Conclusion: The results demonstrated that A. africana crude extract has antimalarial activity both in vitro and in vivo supporting the traditional use of the plant to treat malaria. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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