Inactivation of an Indonesian isolate of foot-and-mouth disease virus using formaldehyde

Autor: Talenta Miracle Tobing, Fedik Abdul Rantam, Thomas Valentinus Widiyatno, Martia Rani Tacharina, Jola Rahmahani, Nusdianto Triakoso, Suryo Kuncorojakti, Heni Puspitasari, Helen Susilowati, Diyantoro Diyantoro, Fadia Azzahra, Yudha Kurniawan, Ahmad Aswin, Edy Budi Susila
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Veterinary World, Vol 17, Iss 6, Pp 1190-1195 (2024)
Druh dokumentu: article
ISSN: 1190-1195
0972-8988
2231-0916
DOI: 10.14202/vetworld.2024.1190-1195
Popis: Background and Aim: Foot-and-mouth disease (FMD) is a highly contagious viral disease that endangers livestock and the environment with significant economic consequences. This study aimed to validate the inactivation of the Indonesian isolate of foot-and-mouth disease virus (FMDV) with various formaldehyde concentration. Materials and Methods: The experiment started with FMDV being adapted on BHK-21 cells until cytopathic effects (CPE) appeared. The biological titer of the virus was determined using the 50% tissue culture infectious dose (TCID50) assay. The virus was inactivated by exposing the isolate to different formaldehyde (FA) concentrations (0.025%, 0.05%, 0.1%, and 0.2%) at 37°C for 24 h, and residual infectivity was assessed using CPE scoring of reinoculated BHK-21 cells. Results: 72 h post-inoculation, the virulence of the FMDV isolate was indicated by complete CPE on BHK-21 monolayer cells, with a TCID50 value of 109/mL; CPE scoring did not signify significant differences (p < 0.05) among 0.025%, 0.05%, 0.1%, 0.2% FA, and the negative control. All treatment groups showed significant differences (p < 0.05) from the positive control (C+). FA concentrations inactivated the FMDV isolate under the given conditions. 0.025% and 0.05% FA continued to display CPE through the third passage, while 0.2% FA did not significantly differ from 0.1% FA (p > 0.05). 0.1% FA is the optimal concentration for safely and effectively inactivating FMDV. Conclusion: All of the formaldehyde concentrations can completely inactivate the FMDV isolate, with the most optimal and safe concentration being 0.1%.
Databáze: Directory of Open Access Journals
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