| Autor: |
Jacob S. Leiby, Kevin McCormick, Scott Sherrill-Mix, Erik L. Clarke, Lyanna R. Kessler, Louis J. Taylor, Casey E. Hofstaedter, Aoife M. Roche, Lisa M. Mattei, Kyle Bittinger, Michal A. Elovitz, Rita Leite, Samuel Parry, Frederic D. Bushman |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Microbiome, Vol 6, Iss 1, Pp 1-11 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2049-2618 |
| DOI: |
10.1186/s40168-018-0575-4 |
| Popis: |
Abstract Background Historically, the human womb has been thought to be sterile in healthy pregnancies, but this idea has been challenged by recent studies using DNA sequence-based methods, which have suggested that the womb is colonized with bacteria. For example, analysis of DNA from placenta samples yielded small proportions of microbial sequences which were proposed to represent normal bacterial colonization. However, an analysis by our group showed no distinction between background negative controls and placenta samples. Also supporting the idea that the womb is sterile is the observation that germ-free mammals can be generated by sterile delivery of neonates into a sterile isolator, after which neonates remain germ-free, which would seem to provide strong data in support of sterility of the womb. Results To probe this further and to investigate possible placental colonization associated with spontaneous preterm birth, we carried out another study comparing microbiota in placenta samples from 20 term and 20 spontaneous preterm deliveries. Both 16S rRNA marker gene sequencing and shotgun metagenomic sequencing were used to characterize placenta and control samples. We first quantified absolute amounts of bacterial 16S rRNA gene sequences using 16S rRNA gene quantitative PCR (qPCR). As in our previous study, levels were found to be low in the placenta samples and indistinguishable from negative controls. Analysis by DNA sequencing did not yield a placenta microbiome distinct from negative controls, either using marker gene sequencing as in our previous work, or with shotgun metagenomic sequencing. Several types of artifacts, including erroneous read classifications and barcode misattribution, needed to be identified and removed from the data to clarify this point. Conclusions Our findings do not support the existence of a consistent placental microbiome, in either placenta from term deliveries or spontaneous preterm births. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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