Anti-proliferating and antioxidant properties of Eriobotrya japonica fruit in human breast cancer cells combined with in silico study

Autor: Astri Arnamalia, Elvan Wiyarta, Reggie Surya, Darmawan Alisaputra, Nurpudji Astuti Taslim, Happy Kurnia Permatasari, Trina Ekawati Tallei, Rosy Iara Maciel Azambuja Ribeiro, Apollinaire Tsopmo, Bonglee Kim, Fahrul Nurkolis, Rony Abdi Syahputra
Jazyk: English<br />Spanish; Castilian
Rok vydání: 2024
Předmět:
Zdroj: CyTA - Journal of Food, Vol 22, Iss 1 (2024)
Druh dokumentu: article
ISSN: 19476337
1947-6345
1947-6337
DOI: 10.1080/19476337.2024.2427242
Popis: This study aimed to identify compounds in the fruits of the medicinal Eriobotrya japonica L, determine anti-apoptotic properties in human breast cancer cells and predict the mechanism via docking and bioinformatic analyses. HPLC-ESI-HRMS/MS metabolite profiling of E. japonica ethanolic extract allowed the identification of eight compounds. Subsequent network pharmacology and molecular docking simulations targeting key cancer-related proteins showed excellent predictive activity (Pa > 0.71) for seven of the compounds. Two of the compounds hyperoxide (C6) and afzelin (C8) showed strong affinities with inducible nitric oxide synthase (iNOS) and beta-1 adrenergic receptor-β (ADRB1) with ΔG of −11.5 and −12.1, respectively. Radical scavenging data showed compound C8 (EC50 89.1 µg/mL, ABTS+•; 96.1 µg/mL, DPPH) was the most active in relation to other compounds and the extract (EC50 115.5 µg/mL, ABTS+•; 101.5 µg/mL, DPPH). The cytotoxicity (LD50) was 96.1–178.9 µg/mL) for cancerous MCF-7 cells compared to 1,105-2,066 µg/mL for normal MCF-10A cells.
Databáze: Directory of Open Access Journals
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