Heterotrophic cultivation of Nannochloropsis salina for enhancing biomass and lipid production

Autor: Thangapandi Marudhupandi, Ramamoorthy Sathishkumar, Thipramalai Thankappan Ajith Kumar
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Biotechnology Reports, Vol 10, Iss C, Pp 8-16 (2016)
Druh dokumentu: article
ISSN: 2215-017X
DOI: 10.1016/j.btre.2016.02.001
Popis: Response surface methodology (RSM) was used to enhance the biomass and lipid content in Nannochloropsis salina due to its economic importance. Preliminary screening results revealed that the heterotrophically cultivated N. salina with various carbon and nitrogen sources yielded higher biomass (0.91 ± 0.0035 g/L) and lipid content (37.1 ± 0.49 mg/L) than that of the photoautotrophical cultivation (0.21 ± 0.009 g/L and 22.16 ± 0.27 mg/L). Significant sources that greatly influenced on biomass and lipid content of the alga were optimized through RSM. The medium consisting of glucose (7.959 g/L), sodium acetate (1.46 g/L), peptone (7.6 g/L) and sodium thiosulphate (1.05 g/L) was found to be the optimal concentration for heterotrophic cultivation by response optimizer. Confirmation experiment results for the RSM optimized concentration yielded the biomass of 1.85 g/L and total lipid content of 48.6 mg/L. In this study, we provide with a strategy for enhancing the biomass and lipid content in N. salina.
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