| Autor: |
Xiaoli Liu, Xiaoou Xue, Hao Wang, Xiaomei Xu |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Journal of Traditional Chinese Medical Sciences, Vol 3, Iss 4, Pp 242-247 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2095-7548 |
| DOI: |
10.1016/j.jtcms.2016.12.008 |
| Popis: |
Objective: To explore the mechanism of the estrogen-like effect of genistein by observing the expression of Matrix metalloproteinases-26 (MMP-26) and ERα in human endometrial cancer cells (Ishikawa and HEC-1B) in vitro. Methods: The effect of genistein on MMP-26 and ERα expression was examined by western blot in cultured Ishikawa and HEC-1B cells. Additionally, the effects of genistein on ERα-ERE-luc and ERβ-ERE-luc reporter gene expression in HEC-1B cells were analyzed by luciferase activity assays. Results: MMP-26 and ERα protein expression was down-regulated by genistein treatment in Ishikawa cell induced by high concentration E2, whereas MMP-26 and ERα protein expression was up-regulated by genistein treatment in Ishikawa cells induced by low concentration E2. Expression of the ERα-ERE-luc and ERβ-ERE-luc reporter genes was significantly increased after E2 induction and was further up-regulated by genistein. Expression of ERα-ERE-luc and ERβ-ERE-luc reporter genes decreased significantly following genistein treatment in high E2 concentrations, and increased significantly following genistein treatment in low E2 concentrations. Conclusions: Genistein showed estrogen-like effects in endometrial cancer cells and influenced estrogen receptor signaling by modulating ERα and ERβ expression. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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