Protocol for quantitative proteomic analysis of heterogeneous adipose tissue-residing progenitor subpopulations in mice

Autor: Bo Shan, Clive S. Barker, Harry Theraulaz, Xiaoli Zhang, Yan Ping, Rana K. Gupta, Mengle Shao, Yibo Wu
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: STAR Protocols, Vol 4, Iss 4, Pp 102676- (2023)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2023.102676
Popis: Summary: Recent studies have revealed cellular heterogeneity of mesenchymal stromal cells and immune cells in adipose tissue and emphasized the need for quantitative analysis of small numbers of functionally distinct cells using state-of-the-art “omics” technologies. Here, we present an optimized protocol for precise protein quantification from minute amounts of samples. We describe steps for isolation of mouse adipose progenitor cells, proteomics sample preparation, mass spectrometry measurement, and computational analysis. This protocol can be adapted to other samples with limited amounts.For complete details on the use and execution of this protocol, please refer to Shan et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Databáze: Directory of Open Access Journals