Kinetic Subpopulations Detected by Single-molecule Spectroscopy: Fundamental Property of Functional Nucleic Acids or Experimental Artefact?
| Autor: | Sebastian L. B. König, Danny Kowerko, Roland K. O. Sigel |
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| Jazyk: | German<br />English<br />French |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | CHIMIA, Vol 67, Iss 4 (2013) |
| Druh dokumentu: | article |
| ISSN: | 0009-4293 2673-2424 |
| DOI: | 10.2533/chimia.2013.240 |
| Popis: | Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1* interaction, two RNA sequences that play an important role in group II intron mediated self-cleavage. Further examples of dynamic heterogeneity in functional nucleic acids are provided and the possible origins of this phenomenon are discussed. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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