Illumina MiSeq sequencing analysis of fungal diversity in stored dates

Autor: Ismail M. Al-Bulushi, Muna S. Bani-Uraba, Nejib S. Guizani, Mohammed K. Al-Khusaibi, Abdullah M. Al-Sadi
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: BMC Microbiology, Vol 17, Iss 1, Pp 1-10 (2017)
Druh dokumentu: article
ISSN: 1471-2180
DOI: 10.1186/s12866-017-0985-7
Popis: Abstract Background Date palm has been a major fruit tree in the Middle East over thousands of years, especially in the Arabian Peninsula. Dates are consumed fresh (Rutab) or after partial drying and storage (Tamar) during off-season. The aim of the study was to provide in-depth analysis of fungal communities associated with the skin (outer part) and mesocarp (inner fleshy part) of stored dates (Tamar) of two cultivars (Khenizi and Burny) through the use of Illumina MiSeq sequencing. Results The study revealed the dominance of Ascomycota (94%) in both cultivars, followed by Chytridiomycota (4%) and Zygomycota (2%). Among the classes recovered, Eurotiomycetes, Dothideomycetes, Saccharomycetes and Sordariomycetes were the most dominant. A total of 54 fungal species were detected, with species belonging to Penicillium, Alternaria, Cladosporium and Aspergillus comprising more than 60% of the fungal reads. Some potentially mycotoxin-producing fungi were detected in stored dates, including Aspergillus flavus, A. versicolor and Penicillium citrinum, but their relative abundance was very limited (
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