Purification of BAC DNA for high-efficiency transgenesis

Autor:	Rajendra K Gangalum, Zhe Jing, Yoshiko Nagaoka, Meisheng Jiang, Suraj P. Bhat
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	BAC DNA transgenesis RECOCHIP Electro-elution Biology (General) QH301-705.5
Zdroj:	BioTechniques, Vol 51, Iss 5, Pp 335-337 (2011)
Druh dokumentu:	article
ISSN:	1940-9818 0736-6205
DOI:	10.2144/000113763
Popis:	An unresolved bottleneck in bacterial artificial chromosome (BAC) transgenesis is low efficiency generation of founder mice because of suboptimal quality of the manipulated BAC DNA. Using mini-gel electrophoresis and electro-elution that circumvents CsCl2 centrifugation, column chromatography, and resin purifications, we have used RECOCHIP, a commercially available dialysis cassette for the purification of BAC DNA that generates transgenic founders with up to 80% efficiency.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/36f103a8aa384b03b9b294c3ae32d2ce Zobrazit plný text záznamu View record in DOAJ