Genetic Relationship Between Gembrong Goat, Kacang Goat and Kacang X Etawah Crossbred (PE) Based on Their Mitochondrial DNA
| Autor: | I Gusti Lanang Oka, Wayan SayangYupardhi, Ida Bagus Mantra, Nyoman Suyasa, Anak Agung Sagung Dewi |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Jurnal Veteriner, Vol 12, Iss 3 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1411-8327 2477-5665 |
| Popis: | Gembrong goat is a specific type of goat which has long hair covers its whole body including its neckand face, originated from eastern part of Bali (Karangasem). A study of this type of goat and its relationshipwith other local goats (Kacang and Kacang x Etawah crossbred) was carried out at Sawe village, Jembrana,Bali.. A number of 12 gembrong goats, 3 kacang goats from Kubu village, Karangasem and 3 Kacang xEtawah crossbred goats from Denpasar were used in this study. Blood samples of all goats were collectedfor mitochondrial DNA (mtDNA) analysis. PCR amplification of D-loop mitochondrial DNA was carriedout by using two primers i.e. CAP-F (5’-CGTGTATGCAAGTACATTAC -3’) and CAP-R (5’-CTGATTAGTCATTAGTCCATC – 3’) . Sequencing of 550 bp (base-pairs) of mitochondrial DNA (productof PCR) only found one polymorphic site at base number 231 with two haplotypes in gembrong goat only,while the other base-pairs were similar between the three goat types (Gembrong, Kacang and Kacang xEtawah crossbred) .The frequency of haplotype 1 was 83.3% and the frequency of haplotype 2 was 16.7%.It was concluded that based on their mitochondrial DNA sequences and “phylogenic analysis”, the threetypes of goat (Gembrong,Kacang and Kacang xEtawah crossbred) had a very close genetic relationship(kinship). |
| Databáze: | Directory of Open Access Journals |
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