Molecular and serological detection of Babesia Bovis and Babesia Bigemina in cattle in the Rio de Janeiro, Brazil

Autor: Jenevaldo Barbosa da Silva, Priscilla Nunes dos Santos, Adivaldo Henrique Fonseca
Jazyk: English<br />Portuguese
Rok vydání: 2014
Předmět:
Zdroj: Semina: Ciências Agrárias, Vol 35, Iss 6, Pp 3139-3146 (2014)
Druh dokumentu: article
ISSN: 1679-0359
1676-546X
DOI: 10.5433/1679-0359.2014v35n6p3139
Popis: Bovine babesiosis is an important disease of cattle where Rhipicephalus microplus acts as a vector for the two causal organisms Babesia bovis and Babesia bigemina. A total of 22 calves were randomly monitored during three years by semi-nested PCR assay and ELISA test to determine prevalence of B. bovis and B. bigemina. The overall prevalence of B. bovis and B. bigemina was 30% and 35% by nested PCR (nPCR), and 70% and 75% by ELISA, respectively. Statistical analysis of the characteristics of animals showed that age and tick infestations (p
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