| Autor: |
St. Elmo Wilken, Susanna Seppälä, Thomas S. Lankiewicz, Mohan Saxena, John K. Henske, Asaf A. Salamov, Igor V. Grigoriev, Michelle A. O’Malley |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Metabolic Engineering Communications, Vol 10, Iss , Pp e00107- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2214-0301 |
| DOI: |
10.1016/j.mec.2019.e00107 |
| Popis: |
Anaerobic fungi (Neocallimastigomycota) are emerging non-model hosts for biotechnology due to their wealth of biomass-degrading enzymes, yet tools to engineer these fungi have not yet been established. Here, we show that the anaerobic gut fungi have the most GC depleted genomes among 443 sequenced organisms in the fungal kingdom, which has ramifications for heterologous expression of genes as well as for emerging CRISPR-based genome engineering approaches. Comparative genomic analyses suggest that anaerobic fungi may contain cellular machinery to aid in sexual reproduction, yet a complete mating pathway was not identified. Predicted proteomes of the anaerobic fungi also contain an unusually large fraction of proteins with homopolymeric amino acid runs consisting of five or more identical consecutive amino acids. In particular, threonine runs are especially enriched in anaerobic fungal carbohydrate active enzymes (CAZymes) and this, together with a high abundance of predicted N-glycosylation motifs, suggests that gut fungal CAZymes are heavily glycosylated, which may impact heterologous production of these biotechnologically useful enzymes. Finally, we present a codon optimization strategy to aid in the development of genetic engineering tools tailored to these early-branching anaerobic fungi. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
