Effect of the supplementation of two capacitive agents in the fertilization medium for the in vitro production of embryos in alpacas (Vicugna pacos)
| Autor: | Walter Palomino-Guerrera, Mijaíl Contreras, Cesar Olaguivel |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | SPERMOVA, Vol 10, Iss 1, Pp 32-37 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2223-9375 2308-4928 |
| DOI: | 10.18548/aspe/0008.05 |
| Popis: | The present study aims to evaluate the effect of heparin and PHE (penicillamine, hypotaurine and epinephrine) in the fertilization medium on the in vitro production of embryos in alpacas. The ovaries were transported in 0.9% physiological saline with gentamicin at 37 ° C for 3 hours. 227 ovaries were used, recovering 1132 oocytes of quality I, II and III; and matured in vitro for 34h. The mature oocytes were randomly distributed in four culture medium treatments, which were supplemented with 2% heparin and PHE according to the following distribution: T1 = Control; T2 = Heparin; T3 = PHE; T4 = PHE + heparin. Then 1x106 sperm/ml selected by the Percoll gradient method (45/90%) and incubated for 18 h. Finally, were cultivated in SOF culture medium for 7 day. The cleavage rate at 24 h in culture were 16.53, 19.92, 21.42 and 23.90 % for T1, T2, T3 and T4, respectively. The group T4 was difference statistically with groups T1, T2 and T3 (P |
| Databáze: | Directory of Open Access Journals |
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