Pairing of homologous chromosomes in C. elegans meiosis requires DEB-1 - an orthologue of mammalian vinculin

Autor: Jana Rohožková, Lenka Hůlková, Jana Fukalová, Petr Flachs, Pavel Hozák
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nucleus, Vol 10, Iss 1, Pp 93-115 (2019)
Druh dokumentu: article
ISSN: 1949-1034
1949-1042
19491034
DOI: 10.1080/19491034.2019.1602337
Popis: During meiosis, homologous chromosomes undergo a dramatic movement in order to correctly align. This is a critical meiotic event but the molecular properties of this ‘chromosomal dance’ still remainunclear. We identified DEB-1 – an orthologue of mammalian vinculin – as a new component of the mechanistic modules responsible for attaching the chromosomes to the nuclear envelope as apart of the LINC complex. In early meiotic nuclei of C. elegans, DEB-1 is localized to the nuclear periphery and alongside the synaptonemal complex of paired homologues. Upon DEB-1 depletion, chromosomes attached to SUN-1 foci remain highly motile until late pachytene. Although the initiation of homologue pairing started normally, irregularities in the formation of the synaptonemal complex occur, and these results in meiotic defects such as increased number of univalents at diakinesis and high embryonic lethality. Our data identify DEB-1 as a new player regulating chromosome dynamics and pairing during meiotic prophase I.
Databáze: Directory of Open Access Journals
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