The Effect of Insulin-like Growth Factor-1, Cysteamine and β-Mercaptoethanol on the In Vitro Maturation of Immature Mice Oocytes
| Autor: | A Dehghan Manshadi, M Rostami Hosseinkhani, M Nikseresht, A Hedayatpoor, M Akbartabar toori, S Mehrabi, R Mahmoudi |
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| Jazyk: | perština |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Armaghane Danesh Bimonthly Journal, Vol 20, Iss 8, Pp 720-731 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1728-6506 1728-6514 |
| Popis: | Background & aim: In vitro maturation of oocytes is a promising technique for reducing the costs and complications of ovarian stimulation by gonadotropins. The aim of this study was to investigate the effects of combination of insulin-like growth factor-1 and antioxidant cysteamine and &beta-Mercaptoethanol on maturation and fertilization of immature oocytes. Methods: in this experimental study, following 48 hrs injection of 7.5 IU PMSG to immature female mice, the germinal vesicle oocytes from ovaries were removed and transferred to TCM199 culture medium containing 50 ng /ml insulin-like growth factor-1 and 100 &mumol Cysteamine and &beta -Mercaptoethanol. After 24 hrs of culture, the oocytes of MII in IVF were fertilized and embryonic development to the two cells was studied under an inverted microscope. Data analysis was performed by using ANOVA and Post hoc Tukey test. Results: The results showed that the rate of maturation, fertilization and 2-cell embryo formation in GV oocytes with cumulus cells in TCM199 medium containing insulin-like growth factor-1, Cysteamine and BME were 92.10, 93.30, 80.60% and in the GV oocytes without Cumulus cells were cultured in the same medium were 65.80, 64.00, 58.60% respectively which were statistically significant (P |
| Databáze: | Directory of Open Access Journals |
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