| Autor: |
Susanna K P Lau, Renate Wernery, Emily Y M Wong, Sunitha Joseph, Alan K L Tsang, Nissy Annie Georgy Patteril, Shyna K Elizabeth, Kwok-Hung Chan, Rubeena Muhammed, Jöerg Kinne, Kwok-Yung Yuen, Ulrich Wernery, Patrick C Y Woo |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Emerging Microbes and Infections, Vol 5, Iss 1, Pp 1-9 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2222-1751 |
| DOI: |
10.1038/emi.2016.129 |
| Popis: |
Little is known regarding the molecular epidemiology of Middle East respiratory syndrome coronavirus (MERS-CoV) circulating in dromedaries outside Saudi Arabia. To address this knowledge gap, we sequenced 10 complete genomes of MERS-CoVs isolated from 2 live and 8 dead dromedaries from different regions in the United Arab Emirates (UAE). Phylogenetic analysis revealed one novel clade A strain, the first detected in the UAE, and nine clade B strains. Strain D998/15 had a distinct phylogenetic position within clade A, being more closely related to the dromedary isolate NRCE-HKU205 from Egypt than to the human isolates EMC/2012 and Jordan-N3/2012. A comparison of predicted protein sequences also demonstrated the existence of two clade A lineages with unique amino acid substitutions, A1 (EMC/2012 and Jordan-N3/2012) and A2 (D998/15 and NRCE-HKU205), circulating in humans and camels, respectively. The nine clade B isolates belong to three distinct lineages: B1, B3 and B5. Two B3 strains, D1271/15 and D1189.1/15, showed evidence of recombination between lineages B4 and B5 in ORF1ab. Molecular clock analysis dated the time of the most recent common ancestor (tMRCA) of clade A to March 2011 and that of clade B to November 2011. Our data support a polyphyletic origin of MERS-CoV in dromedaries and the co-circulation of diverse MERS-CoVs including recombinant strains in the UAE. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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