Microsphere-assistance in microscopic and confocal imaging

Autor: Hüser Lucie, Hagemeier Sebastian, Pahl Tobias, Lehmann Peter
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: EPJ Web of Conferences, Vol 309, p 02014 (2024)
Druh dokumentu: article
ISSN: 2100-014X
DOI: 10.1051/epjconf/202430902014
Popis: Topographical as well as microscopic imaging of nanoscale surfaces plays a pivotal role across various disciplines. Nevertheless, achieving fast, label-free, and accurate characterization of laterally expanded structures below the diffraction limit remains challenging. Recent studies highlight the use of microsphere assistance for resolution improvement. Confocal microscopy, augmented by microspheres, enables the imaging of small structures that were previously inaccessible. This is experimentally compared with microsphere-assisted microscopy (MAM) to underline the decisive role of the confocal effect.
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