Cathepsin B Is Implicated in Triglyceride (TG)-Induced Cell Death of Macrophage
| Autor: | Byung Chul Jung, Jaewon Lim, Sung Hoon Kim, Yoon Suk Kim |
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| Jazyk: | English<br />Korean |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Korean Journal of Clinical Laboratory Science, Vol 52, Iss 3, Pp 245-252 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1738-3544 2288-1662 |
| DOI: | 10.15324/kjcls.2020.52.3.245 |
| Popis: | Macrophage cell death contributes to the formation of plaque, leading to the development of atherosclerosis. The accumulation of triglyceride (TG) is also associated with the pathogenesis of atherosclerosis. A previous study reported that TG induces the cell death of macrophages. This study examined whether the cytoplasmic release of cathepsin B from lysosome is associated with the TG-induced cell death of macrophage. The release of cathepsin B was increased in the TG-treated THP-1 macrophages, but the TG treatment did not affect cathepsin B expression. Furthermore, the inhibition of cathepsin B by its inhibitor, CA-074 Me, partially inhibited the TG-induced cell death of macrophage. TG-triggered macrophage cell death is mediated by the activation of caspase-1, -2, and apoptotic caspases. Therefore, this study investigated whether cathepsin B is implicated in the activation of these caspases. The inhibition of cathepsin B blocked the activation of caspase-7, -8, and -1 but did not affect the activity of caspase-3, -9, and -2. Overall, these results suggest that TG-induced cytoplasmic cathepsin B causes THP-1 macrophage cell death by activating caspase-1, leading to subsequent activation of the extrinsic apoptotic pathway. |
| Databáze: | Directory of Open Access Journals |
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