| Autor: |
Dursun Gözde, Markert Bernd, Stoffel Marcus |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Current Directions in Biomedical Engineering, Vol 5, Iss 1, Pp 171-174 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2364-5504 |
| DOI: |
10.1515/cdbme-2019-0043 |
| Popis: |
Cell-free based approaches are introduced as a promising treatment method for articular cartilage. The success of this method requires cell colonisation from resident tissue into cell-free implants. The objective of our study is to promote the cell colonisation into cell-free collagen I based implants by mechanical stimulation. Therefore, a new in vitro cellular model consisting chondrocyte-seeded matrix and cell-free implants was developed in a polydimethylsiloxan (PDMS) mold. These constructs were cultured under dynamic and static culture conditions. For the dynamic culture, we have developed an in-house bioreactor system where both the load and the deformation applied to the specimen are recorded. Cyclic compression, with a strain of 5% and frequency of 0.1 Hz, was applied to constructs without any break. At the end of three days of dynamic and static cultivation, the cell-free implants were seperated from cell-seeded matrix and cultured in a petri dish three days long. Afterwards, they were analysed using fluoresence dyes. The microscopic assessment indicated that there was a cell migration into the cell-free implants which were cultured dynamically. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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