Autor: |
Caterina Maria Gambino, Luisa Agnello, Anna Maria Ciaccio, Concetta Scazzone, Matteo Vidali, Vincenzo Di Stefano, Salvatore Milano, Filippo Brighina, Giuseppina Candore, Bruna Lo Sasso, Marcello Ciaccio |
Jazyk: |
angličtina |
Rok vydání: |
2023 |
Předmět: |
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Zdroj: |
Journal of Clinical Medicine, Vol 12, Iss 14, p 4781 (2023) |
Druh dokumentu: |
article |
ISSN: |
2077-0383 |
DOI: |
10.3390/jcm12144781 |
Popis: |
The detection of serum anti-acetylcholine receptor (AChR) antibodies is currently an important tool for diagnosing myasthenia gravis (MG) since they are present in about 85% of MG patients. Many serological tests are now available. Nevertheless, results from these tests can be different in some patients. The aim of this study is to compare the sensitivity of a commercially available fixed cell-based assay (F-CBA) to that of enzyme-linked immunosorbent assay (ELISA) kits for anti-AChR detection in patients with a diagnosis of MG. Overall, 143 patients with a confirmed MG diagnosis were included in the study. The detection and measurement of serum anti-AChR antibodies were performed by three analytical methods, namely, a competitive ELISA (cELISA), an indirect ELISA (iELISA), and an F-CBA, according to the manufacturers’ instructions. Anti-AChR antibody titers were positive in 94/143 (66%) using the cELISA, in 75/143 (52%) using the iELISA and in 61/143 (43%) using the F-CBA (adult and/or fetal). Method agreement, evaluated by concordant pairs and Cohen’s kappa, was as follows: cELISA-iELISA: 110/143 (77%), k = 0.53 (95%CI 0.40–0.66); cELISA-F-CBA: 108/143 (76%), k = 0.53 (95%CI 0.41–0.66); iELISA-F-CBA: 121/143 (85%), k = 0.70 (95%CI 0.57–0.80). Our findings show that the cELISA has better analytical performance than the iELISA and F-CBA. However, the iELISA and F-CBA show the highest concordance. |
Databáze: |
Directory of Open Access Journals |
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