| Autor: |
Huanan Wang, Xiangnan Liu, Fanwen Zeng, Tongyuan Zhang, Yuexiao Lian, Miaoli Wu, Li Xiao, Yujun Zhu, Yu Zhang, Meili Chen, Ren Huang, Manlin Luo, Feng Cong, Pengju Guo |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
BMC Veterinary Research, Vol 15, Iss 1, Pp 1-5 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
1746-6148 |
| DOI: |
10.1186/s12917-019-2037-z |
| Popis: |
Abstract Background Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland. Results A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 × 101 copies/μL with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203). Conclusions The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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