| Autor: |
Zhou Zhun, Yuan Han-Wen, Peng Cai-Yun, Jian Yu-Qing, Zhou Xu-Dong, Li Bin, Sheng Wen-Bing, Gong Li-Min, He Shu-Jin, Wang Wei, Liu Chang-Xiao |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Digital Chinese Medicine, Vol 3, Iss 3, Pp 180-187 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2589-3777 |
| DOI: |
10.1016/j.dcmed.2020.09.004 |
| Popis: |
Objective: To explore the major compound in Polygonati Rhizoma (Huang Jing, 黄精) for quality control. Methods: The major compound was isolated and analyzed by liquid chromatography-mass spectrometry (LC-MS), and subsequently further identified by nuclear magnetic resonance (NMR). Thin layer chromatography (TLC) was optimized based on the previous methods reported in the Chinese Pharmacopeia (2015 edition). Results: The major compound was isolated from the natural material and identified as linoleic acid. A high performance liquid chromatography (HPLC) method with robust linearity (R2 = 0.999 7), specificity, precision, stability, repeatability and recovery was developed for linoleic acid determination. TLC chromatogram was improved significantly after optimization for qualitative analysis. Conclusions: The optimized TLC method is practical and can be adopted for quality control of Polygonati Rhizoma (Huang Jing, 黄 精 ). The levels of linoleic acid vary between species of Polygonati Rhizoma (Huang Jing, 黄 精 ), with Polygonatum cyrtonema Hua (Jiang Xing Huang Jing, 姜型黄精) showing the highest contents. This study provides valuable information for quality control of Polygonati Rhizoma (Huang Jing, 黄精). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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