| Autor: |
Baofa Yu, Yan Han, Jian Zhang, Dong Chen |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Frontiers in Nanotechnology, Vol 6 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2673-3013 |
| DOI: |
10.3389/fnano.2024.1465888 |
| Popis: |
ObjectiveNanotechnology offers many advantages in various fields of cancer therapy. This study describes a new method of self-forming nanoparticles (self-nano) using ferric chloride (FeCl₃) and hydrogen peroxide (H2O2) to form the self-nanoparticles in an in vivo tumor. The treatment effect is evaluated.MethodA solution of 3% FeCl3 (0.5 mL) and 1.8% H2O2 (1.0 mL) was injected into the tumor. At various time points post-injection, tumors were collected, and sections were prepared for electron microscopy to evaluate the size of the self-nano particles. Single-cell RNA sequencing (scRNA-seq) was used to analyze the immune changes and their effect on tumor growth.ResultThe formation of self-nano in vitro was observed and confirmed, with particles averaging 421 nm in size for the FeCl3 + H2O2 solution. Over time points ranging from 1 to 14 days, the formed self-nano remained stable at a regular size of 421 ± 8 nm. The self-nano, primarily consisting of iron, induced ferroptosis under the influence of an external magnetic field, leading to tumor growth control through iron-induced cell death and immune reactions. These self-nanoparticles also showed stronger enrichment of pathways related to CD8+ T effect cells (Teff), T cell activation, and regulation of T cell proliferation.ConclusionThe FeCl3 + H2O2 solution can form Fe₂O₃-based self-nanoparticles within tumors through H2O2-incubated oxidation of FeCl3. The self-nano remains effective for over 14 days, inducing ferroptosis and upregulating immune cells under magnetic field treatment. This method offers a novel approach for cancer treatment that can be combined with other modalities. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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