Sympathetic Reinnervation of Intact and Upper Follicle Xenografts into BALB/c-nu/nu Mice

Autor: Xiu-Wen Chen, Na Ni, Xiao-Jun Xie, Ying-Lin Zhao, Wen-Zi Liang, Yu-Xin Huang, Chang-Min Lin
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Life, Vol 13, Iss 11, p 2163 (2023)
Druh dokumentu: article
ISSN: 13112163
2075-1729
DOI: 10.3390/life13112163
Popis: Increasing concerns about hair loss affect people’s quality of life. Recent studies have found that sympathetic nerves play a positive role in regulating hair follicle stem cell activity to promote hair growth. However, no study has investigated sympathetic innervation of transplanted follicles. Rat vibrissa follicles were extracted and implanted under the dorsal skin of BALB/c-nu/nu mice using one of two types of follicles: (1) intact follicles, where transplants included bulbs, and (2) upper follicles, where transplants excluded bulbs. Follicular samples were collected for hematoxylin and eosin staining, immunofluorescence staining for tyrosine hydroxylase (TH, a sympathetic marker) and enzyme-linked immunosorbent assays. At 37 days after implantation in both groups, follicles had entered anagen, with the growth of long hair shafts; tyrosine-hydroxylase-positive nerves were innervating follicles (1.45-fold); and norepinephrine concentrations (2.03-fold) were significantly increased compared to 5 days, but did not return to normal. We demonstrate the survival of intact and upper follicle xenografts and the partial restoration of sympathetic reinnervations of both transplanted follicles.
Databáze: Directory of Open Access Journals
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