| Autor: |
Larissa L.S. Scholte, David J. Leggat, Kristen W. Cohen, Lara Hoeweler, Guacyara C. Erwin, Farhard Rahaman, Angela Lombardo, Vincent Philiponis, Dagna S. Laufer, Heather Siefers, Alexis M. Ruppel, Joshua Brand, Janine Maenza, Rhi Bronson, Madhu Prabhakaran, Jalen Jean-Baptiste, Orpheus Kolokythas, Aimee A. Desrosiers, Caroline K. Thoreson, Antje Heit, Nadia J. Khati, Elissa Malkin, M. Juliana McElrath, Adrian B. McDermott, William R. Schief, David Diemert, Jeffrey M. Bethony |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 4, Iss 4, Pp 102576- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102576 |
| Popis: |
Summary: The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy.For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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