| Autor: |
ZHAI Qingyan, HUO Shengnan, YAO Xianqi, ZHENG Shichao, WANG Jun, REN Yijie, SUN Xiaohui, DU Dongxu |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Zhongguo shipin weisheng zazhi, Vol 35, Iss 3, Pp 360-366 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1004-8456 |
| DOI: |
10.13590/j.cjfh.2023.03.007 |
| Popis: |
ObjectiveTo establish a rapid detection method for adulteration of fur animal-derived components in meat products.MethodsThree positive plasmids （fox， raccon dog and mink） for detection were constructed， and their specificity， sensitivity and applicability were analyzed by real-time quantitative PCR.ResultsThe constructed positive plasmid molecules of fox， raccoon dog and mink had high specificity and sensitivity， and the sensitivity could reach 10-4 ng/μL. The amplification efficiency of standard curves were 94.451%， 117.461% and 114.709%， respectively. And the correlation coefficient R2 was over 0.995. Three components could be detected in mixed meat samples as well as common meat products， the limits of detection were less than 1%， which had good feasibility and applicability.ConclusionThe three positive plasmids constructed by this method could meet the needs of meat adulteration detection in practical work. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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