Detection and quantification by molecular techniques of early infection by Lawsonia intracellularis in suckling piglets

Autor: Víctor Rodriguez-Vega, Héctor Puente, Ana Carvajal, Lucía Pérez-Pérez, Samuel Gómez-Martínez, Fernando L. Leite, Rocío García, Lola Abella, Héctor Argüello
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Porcine Health Management, Vol 10, Iss 1, Pp 1-8 (2024)
Druh dokumentu: article
ISSN: 2055-5660
DOI: 10.1186/s40813-024-00394-6
Popis: Abstract Background Lawsonia intracellularis is the causative agent of Porcine Proliferative Enteropathy (PPE), one of the most prevalent pig enteric diseases worldwide, but with sparse information about early infections in suckling piglets in the epidemiology of PPE. With that aim, this study evaluates the prevalence of L. intracellularis in 3-week-old piglets by analysing ileal digesta content and mucosal scrapings from 383 pigs from 16 farms (aprox., 25 pigs/batch) by real-time qPCR and droplet digital PCR (ddPCR). Results Forty-nine samples yielded a qPCR positive result. Eleven samples from eight farms were confirmed as positive with concentrations of L. intracellularis from 3.5 log10 to 4.5 log10 bacteria/g of sample. Another 16 samples, eight farms, were classified as low positive (2.07–2.38 log10 bacteria/g) and 22 provided an uncertain result. Finally, 334 samples tested negative for L. intracellularis. At batch level, half of the farms included in the study had at least one positive sample and in 10 farms (62.5%) there was at least one low positive sample. The ddPCR was run in 50 of the 383 samples based on their PCR output (including low positive, uncertain and negative samples). Correlation analyses revealed a strong association between qPCR and the ddPCR results (ρ = 0.75; p
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