| Autor: |
Jailson do Nascimento Silva, Amanda Camila dos Santos Linhares, Dario Abel Palmieri, Marcones Ferreira Costa, Gisele Holanda de Sá, Maria Fernanda da Costa Gomes, Michelli Ferreira dos Santos, Lidiane de Lima Feitoza, Sérgio Emílio dos Santos Valente |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Bioscience Journal, Vol 39, Pp e39001-e39001 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1981-3163 |
| DOI: |
10.14393/BJ-v39n0a2023-62577 |
| Popis: |
Molecular markers are important tools in the characterization of plant genetic diversity and can provide support for conservation strategies for endangered populations. The different molecular techniques involve the evaluation of many individuals; therefore, it is crucial to have fast, efficient, and inexpensive methods for DNA extraction. Given the importance of the Aroeira (Myracrodruon urundeuva Fr. All.) it is pertinent to optimize a protocol that allows the obtainment of intact and pure DNA, aiming to assist conservation strategies for this species that is threatened with extinction. Thus, this study aimed to compare five DNA extraction methods: Dellaporta et al. (1983), Doyle and Doyle (1987) modified, Ferreira and Grattapaglia (1995), Romano and Brasileiro (2015), and Khanuja et al. (1999) and optimize the most efficient protocol for M. urundeuva. The modified DNA extraction protocol proposed by Doyle and Doyle (1987), using 100 mg of leaf tissue and 6 µl of β-mercaptoethanol was the protocol that presented the sharpest bands after DNA electrophoresis and after the reactions of amplification employing Polymerase Chain Reaction (PCR). Therefore, it is suggested to use the protocol described by Doyle and Doyle (1987) modified for the extraction of DNA from young M. urundeuva leaves to carry out techniques involving molecular markers. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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