Impact of Wooden Breast myopathy on in vitro protein digestibility, metabolomic profile, and cell cytotoxicity of cooked chicken breast meat

Autor: Thanatorn Trithavisup, Sucheewin Krobthong, Yodying Yingchutrakul, Pornnicha Sanpinit, Yuwares Malila
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Poultry Science, Vol 103, Iss 1, Pp 103261- (2024)
Druh dokumentu: article
ISSN: 0032-5791
DOI: 10.1016/j.psj.2023.103261
Popis: ABSTRACT: This study investigated the impacts of Wooden Breast (WB) abnormality on in vitro protein digestibility and cytotoxicity of cooked chicken breast meat. Chicken breasts without (non-WB, n = 6) or with severe WB condition (WB, n = 6) were cooked and subjected to static in vitro protein digestion. The results showed no significant differences in free-NH2, degree of hydrolysis and distribution of peptide molecular weight between non-WB and WB samples at late intestinal digestion (P5), suggesting no adverse effects of WB on protein digestibility. Based on peptidomic analysis, P5 fraction of WB showed greater content of peptides with oxidative modification than that of non-WB. Untargeted metabolomics did not find any metabolites with potential toxicity either in non-WB and WB. Hydrolyzed non-WB and WB (1.56–100 µg/mL) did not affect viability of Caco-2 and Vero cells but addition of WB samples reduced Caco-2 cell viability compared with non-WB.
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