| Autor: |
Jian-Bo Li, Yong-Feng Luo |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Guoji Yanke Zazhi, Vol 22, Iss 6, Pp 911-915 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1672-5123 |
| DOI: |
10.3980/j.issn.1672-5123.2022.6.05 |
| Popis: |
AIM: To investigate the protective effect and mechanism of ghrelin on human retinal vascular endothelial cells under high glucose.METHODS: A hyperglycemic injury model as a research object was established on human retinal vascular endothelial cells. CCK-8 kit was used to detect the effects of ghrelin with different concentrations on cell proliferation under high glucose, so as to screen the optimal concentration of ghrelin. The cells were then divided into normal control group(NC), ghrelin group(ghrelin), high glucose group(HG)and ghrelin+ high glucose group(ghrelin+HG). Cell proliferation was detected by CCK-8 kit, apoptosis was detected by Annexin-APC/7-AAD kit, and the expressions of NLRP3, Caspase-1, IL-1β and IL-18 proteins were detected by western blotting.RESULTS: Compared with NC group(100.00%±0.00%), the cell proliferation rate of HG group(69.87%±0.68%, P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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