L-Arginine inhibits hydrogen peroxide-induced oxidative damage and inflammatory response by regulating antioxidant capacity in ovine intestinal epithelial cells

Autor: Hao Zhang, Ying Zhang, Xiaoyun Liu, Mabrouk Elsabagh, Yin Yu, Along Peng, Sifa Dai, Hongrong Wang
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Italian Journal of Animal Science, Vol 20, Iss 1, Pp 1620-1632 (2021)
Druh dokumentu: article
ISSN: 1594-4077
1828-051X
1828051X
DOI: 10.1080/1828051X.2021.1973916
Popis: Little is known how L-arginine (Arg) affects the ovine intestinal epithelial cells (IOECs) redox status induced by hydrogen peroxide (H2O2). This study aimed to examine the impact of Arg on IOECs subjected to H2O2-induced oxidative damage, intestinal barrier injury, and inflammatory response. The IOECs were incubated for 16 h then classified as four groups (n = 6/group) and cultured in corresponding media including (1) control (CON) group, in which IOECs were cultured in Arg-free Dulbecco’s modified Eagle’s F12 Ham medium (DMEM) containing 100 μM Arg; (2) Arg group, in which IOECs were cultured in Arg-free DMEM containing 350 μM Arg; (3) H2O2 group, in which IOECs were cultured in CON group plus 150 μM H2O2; (4) Arg + H2O2 group, in which IOECs were cultured in Arg group plus150 μM H2O2. After culturing for 24 h in media, some characteristics of cells in the four groups were measured. Arg administration decreased the H2O2-induced reactive oxygen species (ROS) production compared with the H2O2 group (p
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