Increased autophagy signaling but not proteasome activity in human skeletal muscle after prolonged low‐intensity exercise with negative energy balance

Autor: Marcus Moberg, Gina Hendo, Madelene Jakobsson, C. Mikael Mattsson, Elin Ekblom‐Bak, Mikael Flockhart, Marjan Pontén, Karin Söderlund, Björn Ekblom
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Physiological Reports, Vol 5, Iss 23, Pp n/a-n/a (2017)
Druh dokumentu: article
ISSN: 2051-817X
DOI: 10.14814/phy2.13518
Popis: Abstract Little is known about the molecular regulation of skeletal muscle protein turnover during exercise in field conditions where energy is intake inadequate. Here, 17 male and 7 female soldiers performed an 8 days long field‐based military operation. Vastus lateralis muscle biopsies, in which autophagy, the ubiquitin–proteasome system, and the mTORC1 signaling pathway were studied, were collected before and after the operation. The 187 h long operation resulted in a 15% and 29% negative energy balance as well as a 4.1% and 4.6% loss of body mass in women and men, respectively. After the operation protein levels of ULK1 as well as the phosphorylation of ULK1Ser317 and ULK1Ser555 had increased by 11%, 39%, and 13%, respectively, and this was supported by a 17% increased phosphorylation of AMPKThr172 (P
Databáze: Directory of Open Access Journals
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