| Autor: |
Maryam Imani, Minoo Vahedi Raad, Maliheh Afsari, Farzaneh Fesahat |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
African Journal of Urology, Vol 29, Iss 1, Pp 1-7 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1961-9987 |
| DOI: |
10.1186/s12301-023-00377-z |
| Popis: |
Abstract Background Infertility is a major health problem that affects 7% of the men’s population. Oxidative stress (OS) plays a significant role in the pathophysiology of male infertility. The purpose of this study comparatively evaluated the total anti-oxidation status and DNA/chromatin integrity in semen samples among different infertile men’s groups compared with the normozoospermic men. Methods This cross-sectional study contains four experimental groups, including teratozoospermia (Exp I), asthenoteratozoospermia (Exp II), oligoasthenoteratozoospermia (Exp III), and azoospermia (Exp IV), as well as the control group of normozoospermic men. The total antioxidant capacity (TAC) and total oxidant status (TOS) were assessed by applying the enzyme-linked immunosorbent assay. The chromatin/DNA damage was assessed in semen samples of all study groups by applying chromomycin A3 (CMA3) and toluidine blue (TB) staining methods. Results The results showed significantly higher proportions of TB+ and CMA3 positive sperm in all experimental groups compared to controls (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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