| Autor: |
Jin-Long Yang, Rui Yang, Ke-Fei Shen, Xiang-Wei Peng, Tao Xiong, Zuo-Hua Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Virology Journal, Vol 9, Iss 1, p 47 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1743-422X |
| DOI: |
10.1186/1743-422X-9-47 |
| Popis: |
Abstract Background Sacbrood virus (SBV) primarily infects honeybee broods, and in order to deal with the problem cost effective detection methods are required. Findings A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid identification of SBV. The data demonstrated that, in a simple water bath, SBV RNA could be detected as early as 20 min at 65°C, and a positive amplification reaction was visible to the naked eye due to a color change brought on by the addition of nucleic acid stain SYBR Green. Conclusions The current study presents a method for the rapid and simple detection of SBV by RT-LAMP with high sensitivity and analytic specificity. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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