Superoxide overproduction and kidney fibrosis: a new animal model
| Autor: | Nadia Karina Guimarães-Souza, Liliya Marsovna Yamaleyeva, Baisong Lu, Ana Claudia Mallet de Souza Ramos, Colin Edward Bishop, Karl Erik Andersson |
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| Jazyk: | English<br />Portuguese |
| Předmět: | |
| Zdroj: | Einstein (São Paulo), Vol 13, Iss 1, Pp 79-88 |
| Druh dokumentu: | article |
| ISSN: | 2317-6385 1679-4508 |
| DOI: | 10.1590/S1679-45082015AO3179 |
| Popis: | Objective To establish whether the mutation in the Immp2L gene induces renal fibrosis and whether aging exacerbates renal morphology in mice. Methods Female mutant mice with mutation in the inner mitochondrial membrane peptidase 2-like protein at 3 and 18 months of age were used. Renal fibrosis was analyzed using classic fibrosis score, Masson’s trichrome staining, and analysis of profibrotic markers using real time polymerase chain reaction (superoxide dismutase 1, metalloproteinase-9, erythropoietin, transforming growth factor beta), and immunostaining (fibroblasts and Type IV collagen). Oxidative stress markers were determined by immunohistochemistry. The number of renal apoptotic cells was determined. Renal function was estimated by serum creatinine. Results Young mutant mice had significantly more glomerulosclerosis than age-matched mice (p=0.034). Mutant mice had more tubular casts (p=0.025), collagen deposition (p=0.019), and collagen type IV expression (p |
| Databáze: | Directory of Open Access Journals |
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