| Autor: |
Jannatul Ferdoush Tuli, Mahnaz Ramezanpour, Clare Cooksley, George Spyro Bouras, Kazuhiro Ogi, Sholeh Feizi, Roshan Nepal, Alkis James Psaltis, Peter‐John Wormald, Sarah Vreugde |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Laryngoscope Investigative Otolaryngology, Vol 9, Iss 3, Pp n/a-n/a (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2378-8038 |
| DOI: |
10.1002/lio2.1244 |
| Popis: |
Abstract Introduction In chronic rhinosinusitis (CRS), the congestion and blockage of the nose can cause anaerobic conditions within the sinus cavities which may promote the expression of virulence and antibiotic resistance genes in invading pathogens. Pseudomonas aeruginosa is a facultative anaerobic bacteria and causes severe recalcitrant CRS. In this study, we aimed to evaluate the antimicrobial resistance of P. aeruginosa isolates of CRS patients in planktonic and biofilm form grown in aerobic and anaerobic conditions. Methods P. aeruginosa clinical isolates of CRS patients (n = 25) were grown in planktonic and biofilm form in aerobic and anaerobic conditions. Minimum inhibitory concentrations (MIC) of planktonic forms and minimum biofilm eradication concentrations (MBEC) were determined. Additionally, metabolic activity by fluorescein diacetate assay, biofilm biomass by crystal violet assay and eDNA concentration were assessed in both conditions. Results P. aeruginosa planktonic cells grown in anaerobic condition exhibited increased gentamicin resistance (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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