| Autor: |
Inès Vergez, Magloire Pandoua Nekoua, Cédric Rubrecht, François Fasquelle, Angelo Scuotto, Enagnon Kazali Alidjinou, Didier Betbeder, Didier Hober |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Intervirology, Vol 67, Iss 1, Pp 99-105 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1423-0100 |
| DOI: |
10.1159/000539601 |
| Popis: |
Introduction: The effect of maltodextrin-based nanoparticles with an anionic phospholipid core (lipid-based nanoparticles [NPLs]) on the infection of a human tumoral cell line with poliovirus (PV) has been studied. Methods: NPLs were synthesized and associated with the PV type 1 Sabin strain, and the formulations were characterized. PV and PV/NPL formulations were inoculated to HEp-2 cells. Results: The surface charge and the diameter of PV/NPL formulation suggest that viral particles were adsorbed onto NPLs. When HEp-2 cells were inoculated with 1 tissue culture 50% infectious dose/mL PV associated with NPLs, the cytopathic effect appeared obvious; the levels of the infectious titer of culture supernatants and the proportion of VP1-positive cells were higher. The level of intracellular viral RNA extracted from HEp-2 cells inoculated with PV/NPL formulation was higher as well. Conclusion: These results show that NPLs can enhance the infection with a virus and suggest that they might be used in virotherapy to increase the virus-mediated lysis of tumor cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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